Retinal vascular permeability suppression by topical application of a novel VEGFR2/Src kinase inhibitor in mice and rabbits
J. Clin. Invest. Lea Scheppke, et al. 118:2337 doi:10.1172/JCI33361 [
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Figure 3Src–/– and
Yes–/– mice are resistant to VEGF-induced retinal vascular permeability.
(
A–
C) Whole-mount fluorescent-dextran angiograms in wild-type (
A) and
Src–/– (
B and
C) littermates. Dextran leakage after VEGF injection was more marked in wild-type (
A) than
Src–/– (
C) eyes; vehicle injection was without effect (
B). (
D) In response to VEGF injection, EB accumulation was unaltered in
Src–/– and
Yes–/– retinas but was significantly augmented in
Src and
Yes wild-type littermates. Error bars indicate SEM. PBS/
Yes+/+, 3.07 ± 0.77; VEGF/
Yes+/+, 18.38 ± 5.36,
n = 9; PBS/
Yes–/–, 1.80 ± 0.36; VEGF/
Yes–/–, 1.79+0.24,
n = 10; PBS/
Src–/–, 2.98 ± 0.53; VEGF/
Src–/–, 10.78 ± 2.83,
n = 9; PBS/
Src–/–, 4.61 ± 0.72; VEGF/
Src–/–, 4.67 ± 0.54,
n = 7. Original magnification, ×4. *
P < 0.05.
